CXCL4/PF4 Polyclonal Antibody-BS77247

CXCL4/PF4 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS77247-50, BS77247-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P02776

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200

Background: This gene encodes a member of the CXC chemokine family. This chemokine is released from the alpha granules of activated platelets in the form of a homotetramer which has high affinity for heparin and is involved in platelet aggregation. This protein is chemotactic for numerous other cell type and also functions as an inhibitor of hematopoiesis, angiogenesis and T-cell function. The protein also exhibits antimicrobial activity against Plasmodium falciparum.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 10KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using CXCL4/PF4 antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 180s., Immunohistochemistry of paraffin-embedded rat bone marrow using CXCL4/PF4 Rabbit pAb at dilution of 1:50. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunofluorescence analysis of rat bone marrow using CXCL4/PF4 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: PF-4; CXCL4; SCYB4; PF4

Immunogen: Recombinant fusion protein of human CXCL4/PF4(NP_002610.1).

Conjugate: Unconjugated

Modification: Unmodified