Product Description

Cyclin D1 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66940-50, MB66940-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Activity of the cyclin-dependent kinases CDK4 and CDK6 is regμlated by T-loop phosphorylation, by the abundance of their cyclin partners , and by association with CDK inhibitors of the Cip/Kip or INK family of proteins. The inactive ternary complex of cyclin D/CDK4 and p27 Kip1 requires extracellμlar mitogenic stimμli for the release and degradation of p27 concomitant with a rise in cyclin D levels to affect progression through the restriction point and Rb-dependent entry into S-phas. The active complex of cyclin D/CDK4 targets the retinoblastoma protein for phosphorylation, allowing the release of E2F transcription factors that activate G1/S-phase gene expression. Levels of cyclin D protein drop upon withdrawal of growth factors through downregμlation of protein expression and phosphorylation-dependent degradation .

Category: Primary Antibody

Reactivity: Human, Mouse

Host: Mouse

Applications: WB

Alternate Names: BCL1, PRAD1, G1/S-specific cyclin-D1, B-cell lymphoma 1 protein, BCL-1, BCL-1 oncogene, PRAD1 oncogene

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human Cyclin D1. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of Cyclin D1 protein.

Molecular Weight: ~ 36 kDa

Pathway: Cell Cycle Control G1 S Checkpoin, PI3K AKT signaling

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P24385

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only