Cytokeratin 1 Monoclonal Antibody-MB66054

Cytokeratin 1 Monoclonal Antibody Sizes: 50µl, 100µl Catalogue Numbers: MB66054-50, MB66054-100 Product: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide. Swiss-Prot: P04264 Host: Mouse Reactivity: Human Applications: IHC All Applications: IHC (1/100 - 1/300) Background: Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed as pairs in both keratinized and non-keratinized epithelial tissue. Cytokeratins constitute up to 85% of a mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization, and they function to maintain the overall structural integrity of epithelial cells. The a-helical coiled-coil dimers associate laterally end-to-end to form 10-nm diameter filaments. Cytokeratins are useful markers of tissue differentiation, and they aid in the characterization of malignant tumors. Cytokeratin 1 is highly expressed in several malignancies including epithelioid hemangioendohtheliomas, angiosarcomas, schwannomas, epithelioid sarcomas and synodal sarcomas. The gene encoding human Cytokeratin 1 maps to chromosome 12q13.13. Mutations in the gene encoding human Cytokeratin 1 lead to abnormal filament associations and epidermolytic hyperkeratosis. Purification and Purity: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE). Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Extra Notes: Immunohistochemical analysis of Cytokeratin 1 staining in human skin formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6. 0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Bioworld Molecular Weight: ~ 66 kDa Specificity: Recognizes endogenous levels of Cytokeratin 1 protein. Alternative Name: KRTA; Keratin type II cytoskeletal 1; 67 kDa cytokeratin; Cytokeratin-1; CK-1; Hair alpha protein; Keratin-1; K1; Type-II keratin Kb1 Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Cytokeratin 1. The exact sequence is proprietary. Conjugate: Unconjugated Modification: Unmodified