Product Description

Cytokeratin 17 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66058-50, MB66058-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Keratins (cytokeratins) are intermediate filament proteins that are mainly expressed in epithelial cells. Keratin heterodimers composed of an acidic keratin (or type I keratin, keratins 9 to 23) and a basic keratin (or type II keratin, keratins 1 to 8) assemble to form filaments. Keratin isoforms demonstrate tissue- and differentiation-specific profiles that make them usefμl as research biomarkers. Research studies have shown that mutations in keratin genes are associated with skin disorders, liver and pancreatic diseases, and inflammatory intestinal diseases. Keratin 17 is involved in wound healing and cell growth, two processes that require rapid cytoskeletal remodeling. Keratinocytes deficient in keratin 17 exhibit abnormal Akt/mTOR signaling and fail to produce an increase in translation, cell size, or growth, these cells also exhibit abnormal 14-3-3σ localization. As 14-3-3σ typically associates with keratin 17, these resμlts imply that Akt/mTOR signaling resμlts in sequestration of 14-3-3σ with keratin 17 in the cytosol, which is required for translation and cell growth. Phosphorylation of keratin 17 on Ser44 may provide a docking site for 14-3-3σ binding.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IHC

Alternate Names: Keratin type I cytoskeletal 17, 39.1, Cytokeratin-17, CK-17, Keratin-17, K17

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within human Cytokeratin 17. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IHC (1/100 - 1/300)

Purification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of Cytokeratin 17 protein.

Molecular Weight: ~ 51 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q04695

Product: Mouse IgG1. Liquid in PBS containing 50% glycerol, 0.2% BSA and 0.01% sodium azide.

Research Use Only