Cytokeratin 19 Monoclonal Antibody-MB66849

Cytokeratin 19 Monoclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: MB66849-50, MB66849-100

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Swiss-Prot: P08727

Host: Mouse

Reactivity: Human

Applications: WB, IHC, IF/ICC

All Applications: WB (1/1000 - 1/4000), IHC (1/50 - 1/200)

Background: Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

Purification and Purity: This antibody is purified through a protein G column.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Recognizes endogenous levels of Cytokeratin 19 protein.

Bioworld Molecular Weight: ~ 48 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of Cytokeratin 19 expression in HepG2 (A), MCF7 (B) whole cell lysates., Immunohistochemical analysis of Cytokeratin 19 staining in human colon carcinoma formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX., Immunofluorescent analysis of Cytokeratin 19 staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Alternative Name: Keratin, type I cytoskeletal 19; Cytokeratin-19; CK-19; Keratin-19; K19

Immunogen: Recombinant fusion protein of human Cytokeratin 19. The exact sequence is proprietary.

Conjugate: Unconjugated

Modification: Unmodified