Product Description

Cytokeratin 8 (phospho Ser432) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES6030-50, ES6030-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: keratin 8(KRT8) Homo sapiens This gene is a member of the type II keratin family clustered on the long arm of chromosome 12. Type I and type II keratins heteropolymerize to form intermediate-sized filaments in the cytoplasm of epithelial cells. The product of this gene typically dimerizes with keratin 18 to form an intermediate filament in simple single-layered epithelial cells. This protein plays a role in maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation. Mutations in this gene cause cryptogenic cirrhosis. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jan 2012],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: KRT8, CYK8, Keratin, type II cytoskeletal 8, Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human Keratin 8 around the phosphorylation site of Ser432. AA range:401-450

Isotype: IgG

Target: Cytokeratin 8

Specificity: Phospho-Cytokeratin 8 (S432) Polyclonal Antibody detects endogenous levels of Cytokeratin 8 protein only when phosphorylated at S432.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin, Expressed in gingival mucosa and hard palate of the oral cavity,

Observed Band (KD): 53kD

Function: Disease: Defects in KRT8 are a cause of cryptogenic cirrhosis [MIM: 215600]
Function: Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle
Miscellaneous: There are two types of cytoskeletal and microfibrillar keratin: I (acidic, 40-55 kDa) and II (neutral to basic, 56-70 kDa)
PTM: O-glycosylated at multiple sites, glycans consist of single N-acetylglucosamine residues
PTM: Phosphorylation on serine residues is enhanced during EGF stimulation and mitosis. Ser-74 phosphorylation plays an important role in keratin filament reorganization
Similarity: Belongs to the intermediate filament family
Subunit: Heterotetramer of two type I and two type II keratins. keratin-8 associates with keratin-18. Associates with KRT20. Interacts with HCV core protein and PNN. When associated with KRT19, interacts with DMD. Interacts with TCHP
tissue spec

Subcellular Location: Cytoplasm Nucleus, nucleoplasm Nucleus matrix

Storage and Stability: -20°C/1 year

Gene Name: KRT8

Protein Name: Keratin type II cytoskeletal 8

Mouse Gene ID: 16691

Mouse SwissProt Number: P11679

Rat Gene ID: 25626

Rat SwissProt Number: Q10758

Research Use Only