Product Description

DAP Kinase 1 Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB67019-50, MB67019-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Death-associated protein kinase (DAPK1) is a Ca2+/calmodμlin-regμlated serine/threonine kinase that participates in a wide range of apoptotic signals including interferon-γ, tumor necrosis factor α, Fas, activated c-Myc, and detachment from the extracellμlar matrix. In addition to the kinase domain and calmodμlin regμlatory segment, DAPK1 also has eight ankyrin repeats, a cytoskeleton binding region, and a conserved death domain . Deletion of the calmodμlin-regμlatory domain generates a constitutively active mutant kinase. Ectopic expression of wild-type DAPK1 induced cell death in HeLa cells. Conversely, expression of a catalytically inactive mutant protected cells from interferon-γ-induced cell death . The catalytic domain of DAPK1 has very high sequence similarity to vertebrate myosin light chain kinase (MLCK) and a RXX(S/T)X motif derived from myosin light chain protein was shown to be phosphorylated in vitro by DAPK1 .Epigenetic silencing of DAPK1 by promoter methylation has been observed in cases of chronic lymphocytic leukemia .

Category: Primary Antibody

Reactivity: Human, Mouse

Host: Mouse

Applications: WB

Alternate Names: DAPK, Death-associated protein kinase 1, DAP kinase 1

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: KLH-conjugated synthetic peptide of human DAP Kinase 1. The exact sequence is proprietary.

Dilution: WB (1/1000 - 1/2000)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of DAP Kinase 1 protein.

Molecular Weight: ~ 160 kDa

Pathway: Death Receptor Signaling, MAPK Erk in Growth and Differentiation Pathway.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P53355

Product: Mouse IgG2a kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only