Product Description

DAP Kinase 1 (Phospho-S308) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS67434-50, BS67434-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Death-associated protein kinase (DAPK1) is a Ca2+/calmodμlin-regμlated serine/threonine kinase that participates in a wide range of apoptotic signals including interferon-γ, tumor necrosis factor α, Fas, activated c-Myc, and detachment from the extracellμlar matrix. In addition to the kinase domain and calmodμlin regμlatory segment, DAPK1 also has eight ankyrin repeats, a cytoskeleton binding region, and a conserved death domain . Deletion of the calmodμlin-regμlatory domain generates a constitutively active mutant kinase. Ectopic expression of wild-type DAPK1 induced cell death in HeLa cells. Conversely, expression of a catalytically inactive mutant protected cells from interferon-γ-induced cell death . The catalytic domain of DAPK1 has very high sequence similarity to vertebrate myosin light chain kinase (MLCK) and a RXX(S/T)X motif derived from myosin light chain protein was shown to be phosphorylated in vitro by DAPK1 .Epigenetic silencing of DAPK1 by promoter methylation has been observed in cases of chronic lymphocytic leukemia.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat, Pig

Host: Rabbit

Applications: WB

Alternate Names: DAPK, Death-associated protein kinase 1, DAP kinase 1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human DAP Kinase 1 with a site at pS308. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of DAP Kinase 1 with a site at pS308 protein.

Molecular Weight: ~ 160 kDa

Pathway: Death Receptor Signaling, MAPK Erk in Growth and Differentiation Pathway(.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P53355

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only