Product Description

Daxx (phospho Ser668) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4968-50, ES4968-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a multifunctional protein that resides in multiple locations in the nucleus and in the cytoplasm. It interacts with a wide variety of proteins, such as apoptosis antigen Fas, centromere protein C, and transcription factor erythroblastosis virus E26 oncogene homolog 1. In the nucleus, the encoded protein functions as a potent transcription repressor that binds to sumoylated transcription factors. Its repression can be relieved by the sequestration of this protein into promyelocytic leukemia nuclear bodies or nucleoli. This protein also associates with centromeres in G2 phase. In the cytoplasm, the encoded protein may function to regulate apoptosis. The subcellular localization and function of this protein are modulated by post-translational modifications, including sumoylation, phosphorylation and polyubiquitination. Alternative splicing results in multiple transcript varian

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: DAXX, BING2, DAP6, Death domain-associated protein 6, Daxx, hDaxx, ETS1-associated protein 1, EAP1, Fas death domain-associated protein

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human Daxx around the phosphorylation site of Ser668. AA range:634-683

Isotype: IgG

Target: Daxx

Specificity: Phospho-Daxx (S668) Polyclonal Antibody detects endogenous levels of Daxx protein only when phosphorylated at S668.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Ubiquitous,

Observed Band (KD): 85-115kd

Fields: MAPK signaling pathway, Apoptosis, Parkinson disease, Amyotrophic lateral sclerosis, Pathways of neurodegeneration - multiple diseases, Herpes simplex virus 1 infection

Function: Function: Proposed to mediate activation of the JNK pathway and apoptosis via MAP3K5 in response to signaling from TNFRSF6 and TGFBR2. Interaction with HSPB1/HSP27 may prevent interaction with TNFRSF6 and MAP3K5 and block DAXX-mediated apoptosis. In contrast, in lymphoid cells JNC activation and TNFRSF6-mediated apoptosis may not involve DAXX. Seems to regulate transcription in PML/POD/ND10 nuclear bodies together with PML and may influence TNFRSF6-dependent apoptosis thereby. Down-regulates basal and activated transcription. Seems to act as a transcriptional co-repressor and inhibits PAX3 and ETS1 through direct protein-protein interaction. Modulates PAX5 activity. Its transcription repressor activity is modulated by recruiting it to subnuclear compartments like the nucleolus or PML/POD/ND10 nuclear bodies through interactions with MCSR1 and PML, respectively
Induction: Upon mitogenic st

Subcellular Location: Cytoplasm Nucleus, nucleoplasm Nucleus, PML body Nucleus, nucleolus Chromosome, centromere Dispersed throughout the nucleoplasm, in PML/POD/ND10 nuclear bodies, and in nucleoli (Probable) Colocalizes with histone H33, ATRX, HIRA and ASF1A at PML-nuclear bodies (PubMed:12953102, PubMed:14990586, PubMed:23222847, PubMed:24200965) Colocalizes with a subset of interphase centromeres, but is absent from mitotic centromeres (PubMed:9645950) Detected in cytoplasmic punctate structures (PubMed:11842083) Translocates from the nucleus to the cytoplasm upon glucose deprivation or oxidative stress (PubMed:12968034) Colocalizes with RASSF1 in the nucleus (PubMed:18566590) Colocalizes with USP7 in nucleoplasma with accumulation in speckled structures (PubMed:16845383), [Isoform beta]

Storage and Stability: -20°C/1 year

Gene Name: DAXX

Protein Name: Death domain-associated protein 6

Mouse SwissProt Number: O35613

Research Use Only