Product Description

DBH Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4969-50, ES4969-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene is an oxidoreductase belonging to the copper type II, ascorbate-dependent monooxygenase family. It is present in the synaptic vesicles of postganglionic sympathetic neurons and converts dopamine to norepinephrine. It exists in both soluble and membrane-bound forms, depending on the absence or presence, respectively, of a signal peptide. [provided by RefSeq, Jul 2008],

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, ELISA

Alternate Names: DBH, Dopamine beta-hydroxylase, Dopamine beta-monooxygenase

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human DBH. AA range:401-450

Isotype: IgG

Target: DBH

Specificity: DBH Polyclonal Antibody detects endogenous levels of DBH protein.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Brain, Plasma,

Observed Band (KD): 69kD

Fields: Tyrosine metabolism, Metabolic pathways

Function: Catalytic Activity: 3,4-dihydroxyphenethylamine + ascorbate + O(2) = noradrenaline + dehydroascorbate + H(2)O
Cofactor: Binds 1 PQQ per subunit
Cofactor: Binds 2 copper ions per subunit
Disease: Defects in DBH are the cause of DBH deficiency [MIM: 223360], also called norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction
Function: Conversion of dopamine to noradrenaline
Induction: Activity is enhanced by nerve growth factor (in superior cervical ganglia and adrenal medulla). Trans-synaptic stimulation with reserpine, acetylcholine and glucocorticoids
Online Information: Dopamine beta hydroxylase entry,Pathway: Catecholamine biosynthesis, norepinephrine biosynthesis, norepinephrine from dopamine: step 1/1
polymorphism:

Subcellular Location: [Soluble dopamine beta-hydroxylase]: Cytoplasmic vesicle, secretory vesicle lumen Cytoplasmic vesicle, secretory vesicle, chromaffin granule lumen Secreted, Cytoplasmic vesicle, secretory vesicle membrane, Single-pass type II membrane protein Cytoplasmic vesicle, secretory vesicle, chromaffin granule membrane, Single-pass type II membrane protein

Storage and Stability: -20°C/1 year

Gene Name: DBH

Protein Name: Dopamine beta-hydroxylase

Mouse SwissProt Number: Q64237

Research Use Only