DDDDK-tag Polyclonal Antibody-AP0007

DDDDK-tag Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: AP0007-50, AP0007-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Host: Rabbit

Reactivity: All

Applications: WB, IP, IF

All Applications: WB: 1:2000~1:5000
IP: 1:50~1:200
IF: 1:50~1:200

Background: Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation and immunostaining techniques. Due to their small size, they are unlikely to affect the tagged protein's biochemical properties.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: DDDDK-tag polyclonal antibody detects over-expressed or recombinant proteins containing the DDDDK epitope tag.

Bioworld Molecular Weight: N/A

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of DDDDK-tag pAb at 1:5000 dillution
Lane 1: HEK293T whole cell lysate
Lane 2: HEK293T whole cell lysate,transfected with pcDNA3.1-DDDDK-p62-HA., IF image of AP0007 stained HEK293T cells, transfected with pcDNA3.1-HA-DDDDK-p62 #PPL00549-2b. The cells were 4% paraformaldehyde fixed (20 min) and then incubated in 10% normal goat serum for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody DDDDK-tag pAb #AP0007(1:200) and the antibody HA-tag (4G3) mAb #AP0005M(1:200) at 5µg/ml overnight at +4°C. The secondary antibody (Green) wasGoat anti-Rabbit IgG (H+L) -FITC#BS10950 and the secondary antibody (Red) was Goat anti-Mouse IgG (H+L)-TRITC#BS11502. Hoechst33342 #BD5011 was used to stain the cell nuclei (blue)., Immunoprecipitation of 293F cell transfected with pcDNA3.4-TfR-DDDDK lysates using DDDDK-Tag pAb (Sepharose Bead Conjugate)#BD0048 (Lane 2 and Lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate)#BD0048 (Lane 4 and Lane 5). Lane 1 is 30% input. The western blot was probed using DDDDK-Tag pAb #AP0007.

Alternative Name: DDDDK epitope tag; DYKDDDDK epitope tag; DYKDDDDK tag; ECS epitope tag,ECS tag; Enterokinase Cleavage Site epitope tag; Enterokinase Cleavage Site tag; DDDDK

Immunogen: Synthetic peptide DDDDK-tag

Conjugate: Unconjugated

Modification: Unmodified