Product Description

DDIT3/CHOP Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS79646-50, BS79646-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P35638

Host: Rabbit

Reactivity: Mouse, Rat

Applications: WB, IHC, IP,

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IP,1:100 - 1:500

Background: This gene encodes a member of the CCAAT/enhancer-binding protein (C/EBP) family of transcription factors. The protein functions as a dominant-negative inhibitor by forming heterodimers with other C/EBP members, such as C/EBP and LAP (liver activator protein), and preventing their DNA binding activity. The protein is implicated in adipogenesis and erythropoiesis, is activated by endoplasmic reticulum stress, and promotes apoptosis. Fusion of this gene and FUS on chromosome 16 or EWSR1 on chromosome 22 induced by translocation generates chimeric proteins in myxoid liposarcomas or Ewing sarcoma. Multiple alternatively spliced transcript variants encoding two isoforms with different length have been identified.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 30KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of C6 cells, using DDIT3/CHOP antibody at 1:1000 dilution.C6 cells were treated by tunicamycin for 8 hours.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunohistochemistry of paraffin-embedded mouse spleen using DDIT3/CHOP Rabbit pAb at dilution of 1:200. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded rat spleen using DDIT3/CHOP Rabbit pAb at dilution of 1:200. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunoprecipitation analysis of 300ug extracts of C2C12 tunicamycin cells using 3ug DDIT3/CHOP antibody. Western blot was performed from the immunoprecipitate using DDIT3/CHOP antibody at a dilution of 1:500.

Alternative Name: DDIT3; CEBPZ; CHOP; CHOP-10; CHOP10; GADD153; C/EBPzeta; DDIT3 / CHOP

Immunogen: Recombinant fusion protein of human DDIT3/CHOP(NP_004074.2).

Conjugate: Unconjugated

Modification: Unmodified