Product Description

DDX58 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES11056-50, ES11056-100

Lead times: approximately 7-10 business days, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases which are implicated in a number of cellular processes involving RNA binding and alteration of RNA secondary structure. This gene encodes a protein containing RNA helicase-DEAD box protein motifs and a caspase recruitment domain (CARD). It is involved in viral double-stranded (ds) RNA recognition and the regulation of immune response. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB, ELISA

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from part region of human protein

Isotype: IgG

Target: DDX58,

Specificity: DDX58 Polyclonal Antibody detects endogenous levels of protein.

Dilution: WB 1:500-2000 ELISA 1:5000-20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formulation: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Expression: Present in vascular smooth cells (at protein level).

Observed Band (KD): 101kD

Fields: NF-kappa B signaling pathway, RIG-I-like receptor signaling pathway, Cytosolic DNA-sensing pathway, Hepatitis C, Hepatitis B, Measles, Influenza A, Herpes simplex virus 1 infection, Epstein-Barr virus infection, Coronavirus disease - COVID-19

Function: Domain: The 2 CARD domains are responsible for interaction with and signaling through MAVS. Domain: The helicase domain is responsible for dsRNA recognition. Domain: The repressor domain controls homomultimerization and interaction with MAVS. Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. induction:By bacterial lipopolysaccharide (LPS) in endothelial cells. By IFN-alpha, -beta and -gamma. PTM: Isgylated. Conjugated to ubiquitin-like protein ISG15

Subcellular Location: Cytoplasm. Cell projection, ruffle membrane. Cytoplasm, cytoskeleton. Cell junction, tight junction. Colocalized with TRIM25 at cytoplasmic perinuclear bodies. Associated with the actin cytoskeleton at membrane ruffles.

Storage and Stability: -20°C/1 year

Gene Name: DDX58

Protein Name: Probable ATP-dependent RNA helicase DDX58, EC 3.6.4.13, DEAD box protein 58, RIG-I-like receptor 1, RLR-1, Retinoic acid-inducible gene 1 protein, RIG-1, Retinoic acid-inducible gene I protein

Human Gene ID: 23586

Human SwissProt Number: O95786

Mouse SwissProt Number: Q6Q899

Research Use Only