DGK-θ (S725) Polyclonal Antibody-BS2479

DGK-θ (S725) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS2479-50, BS2479-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P52824

Host: Rabbit

Reactivity: Human

Applications: IHC

All Applications: IHC: 1:50~1:200

Background: Diacylglycerol (DAG) is a fundamental lipid second messenger that is produced in the nucleus. The accumulation of DAG in the nucleus is important for the regulation of cell growth and differentiation. Diacylglycerol kinases (DGKs) convert DAG to phosphatidic acid, thereby terminating diacylglycerol signaling, which results in the reduction of protein kinase C activity and cell cycle progression of T lymphocytes. Diacylglycerol kinases are divided into five subtypes, Type I-Type V. DGK-θ is a Type V DGK, and localizes mainly to the nucleus of various cell lines, such as MDA-MB-453, MCF-7, PC12 and HeLa. Nuclear DGK-θ co-localizes with phosphatidylinositol 4,5-bisphosphate (PIP(2)). DGK-θ is the isoform responsive to α-Thrombin stimulation.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of DGK-θ (S725) pAb in paraffin-embedded human colorectal carcinoma tissue at 1:50.

Bioworld Molecular Weight: ~ 101 kDa

Specificity: Diacylglycerol (DAG) is a fundamental lipid second messenger that is produced in the nucleus. The accumulation of DAG in the nucleus is important for the regulation of cell growth and differentiation. Diacylglycerol kinases (DGKs) convert DAG to phosphati

Alternative Name: DGKQ; DAGK4; Diacylglycerol kinase theta; DGK-theta

Immunogen: Synthetic peptide, corresponding to amino acids 700-750 of Human DGK-θ.

Conjugate: Unconjugated

Modification: Unmodified