Product Description

DHX36 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: AP6004-50, AP6004-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q9H2U1

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC

All Applications: WB: 1:500~1:1000
IHC: 1:50~1:200

Background: DDX36 (DEAH box protein 36), also known as MLE-like protein 1 and RNA helicase associated with AU-rich element ARE, is a 1,008 amino acid protein that is expressed in testis and may function in sex development and spermatogenesis.DDX36 plays a role in degradation and deadenylation of mRNAs that contain the consensus ARE sequence element in their 3'-UTR. There are three isoforms of DDX36 that exist as a result of alternative splicing events.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: DHX36 polyclonal antibody detects endogenous levels of DHX36 protein.

Bioworld Molecular Weight: ~ 115 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of DHX36 polyclonal antibody at 1:500 dilution
Lane 1: PC3 whole cell lysate (40ug)
Lane 2: L02 whole cell lysate (20ug)
Lane 3: HEK293T whole cell lysate (20ug)
Lane 4: The liver tissue lysate of Mouse (40ug)
Lane 5: The liver tissue lysate of Rat (40ug), Immunohistochemistry (IHC) analyzes of DHX36 pAb in paraffin-embedded human breast carcinoma tissue at 1:50, showing cytoplasm and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase., Immunohistochemistry (IHC) analyzes of DHX36 pAb in paraffin-embedded human kidney carcinoma tissue at 1:50, showing cytoplasm and nucleus staining. Negative control (the right) Using PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG-biotin followed by avidin-peroxidase.

Alternative Name: ATP-dependent RNA helicase DHX36; DEAH box protein 36; G4-resolvase 1; G4R1; MLE-like protein 1; RNA helicase associated with AU-rich element ARE; DHX36; DDX36; KIAA1488; MLEL1; RHAU

Immunogen: Recombinant full length Human DHX36.

Conjugate: Unconjugated

Modification: Unmodified