Product Description

DIAPH1 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS7851-50, BS7851-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Dia 1 (also designated p140mDia) and Dia 2 (also designated p134mDia 2), mammalian homologs of Drosophila Diaphanous, belong to a family of formin-homology (FH) proteins, which are characterized by having tandemly aligned FH1 and FH2 domains in their carboxy terminal regions. The amino terminus of inactivated Dia 1 binds to the FH1 and FH2 domains, and is activated when GTP-bound Rho disrupts the intramolecμlar interaction of Dia 1 and converts it to an open conformation. Once activated, Dia 1 co-localizes with profilin and RhoA in dynamic plasma-membrane structures, and induces the formation of stress fibers in conjunction with the ROCK mediated signaling pathway. However, Dia 1 and ROCK activities antagonize each other in order to determine the thickness and density of the stress fibers formed. Dia 1 has also been implicated as the gene responsible for non-syndromic deafness.

Category: Primary Antibody

Reactivity: Human, Mouse

Host: Rabbit

Applications: WB, IF

Alternate Names: DIAPH1, deafness, autosomal dominant 1, DFNA1, DIA1, DIAP1, DIAP1_HUMAN, DIAPH1, Diaphanous homolog 1 (Drosophila), diaphanous homolog 1, Diaphanous related formin 1, Diaphanous-related formin-1, DRF1, FLJ25265, hDIA1, LFHL1, low frequency hearing loss 1, p140DIA, Protein diaphanous homolog 1,

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fμll length Human DIAPH1.

Dilution: WB 1:500 - 1:2000
IF 1:50 - 1:100

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: DIAPH1 polyclonal antibody detects endogenous levels of DIAPH1 protein.

Molecular Weight: ~ 178kDa

Pathway: Adherens Junction signaling, Death Receptor Signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: O60610

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only