DIO3 Polyclonal Antibody - E-AB-92745

DIO3 Polyclonal Antibody

Sizes: 60μL, 120μL, 200μL

Catalogue Numbers: E-AB-92745-60, E-AB-92745-120, E-AB-92745-200

Citations, Manuals and MSDS Available upon request.

Abbreviation: DIO3

Target Synonym: DIO3; 5DIII; D3; DIOIII; TXDI3

Research Areas: Cancer, Metabolism, Neuroscience, Signal Transduction

Conjugation: Unconjugated

Host: Rabbit

Species Reactivity: Human

Application: WB

Isotype: IgG

Clonality: Polyclonal

UNIProt ID: P55073

Background: The protein encoded by this intronless gene belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3, 3', 5-triiodothyronine (T3) to inactive metabolites, 3, 3', 5'-triiodothyronine (RT3) and 3, 3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in pregnant uterus, placenta, fetal and neonatal tissues, and thought to prevent premature exposure of developing fetal tissues to adult levels of thyroid hormones. It regulates circulating fetal thyroid hormone concentrations, and thus plays a critical role in mammalian development. Knockout mice lacking this gene exhibit abnormalities related to development and reproduction, and increased activity of this enzyme in infants with hemangiomas causes severe hypothyroidism. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal.

Concentration: 1mg/mL

Immunogen: Recombinant fusion protein of human DIO3

Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Purification Method: Affinity purification

Dilution: WB 1:500-1:2000

Calculated MW: 33kDa

Observed MW: 34kDa

Storage: -20°C/One year. Avoid freeze / thaw cycles.

Shipping: Ice bag

Research Use Only