Product Description

DIO3 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES2173-50, ES2173-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this intronless gene belongs to the iodothyronine deiodinase family. It catalyzes the inactivation of thyroid hormone by inner ring deiodination of the prohormone thyroxine (T4) and the bioactive hormone 3,3',5-triiodothyronine (T3) to inactive metabolites, 3,3',5'-triiodothyronine (RT3) and 3,3'-diiodothyronine (T2), respectively. This enzyme is highly expressed in pregnant uterus, placenta, fetal and neonatal tissues, and thought to prevent premature exposure of developing fetal tissues to adult levels of thyroid hormones. It regulates circulating fetal thyroid hormone concentrations, and thus plays a critical role in mammalian development. Knockout mice lacking this gene exhibit abnormalities related to development and reproduction, and increased activity of this enzyme in infants with hemangiomas causes severe hypothyroidism. This protei

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: DIO3, ITDI3, TXDI3, Type III iodothyronine deiodinase, 5DIII, DIOIII, Type 3 DI, Type-III 5'-deiodinase

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human DIO3. AA range:17-66

Isotype: IgG

Target: DIO3

Specificity: DIO3 Polyclonal Antibody detects endogenous levels of DIO3 protein.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Expressed in placenta and several fetal tissues,

Observed Band (KD): 31kD

Fields: Thyroid hormone signaling pathway

Function: Catalytic Activity: 3,3',5'-triiodo-L-thyronine + iodide + A + H(+) = L-thyroxine + AH(2)
Function: Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development
Function: Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine)
Similarity: Belongs to the iodothyronine deiodinase family
Tissue Specificity: Expressed in placenta and several fetal tissues

Subcellular Location: Cell membrane, Single-pass type II membrane protein Endosome membrane, Single-pass type II membrane protein

Storage and Stability: -20°C/1 year

Gene Name: DIO3

Protein Name: Type III iodothyronine deiodinase

Mouse Gene ID: 107585

Mouse SwissProt Number: Q91ZI8

Rat Gene ID: 29475

Rat SwissProt Number: P49897

Research Use Only