Product Description

DLDH/DLD Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS7282-50, BS7282-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P09622

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200

Background: This gene encodes a member of the class-I pyridine nucleotide-disulfide oxidoreductase family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. In homodimeric form, the encoded protein functions as a dehydrogenase and is found in several multi-enzyme complexes that regulate energy metabolism. However, as a monomer, this protein can function as a protease. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. Alternative splicing results in multiple transcript variants.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 54kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using DLDH/DLDH/DLDH/DLD antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s., Immunohistochemistry of paraffin-embedded human colon carcinoma using DLDH/DLDH/DLDH/DLD Rabbit pAb at dilution of 1:500. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded human placenta using DLDH/DLDH/DLDH/DLD Rabbit pAb at dilution of 1:500. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunofluorescence analysis of HeLa cells using DLDH/DLDH/DLDH/DLD antibody. Blue: DAPI for nuclear staining.

Alternative Name: DLD; DLDD; DLDH; E3; GCSL; LAD; PHE3

Immunogen: Recombinant fusion protein of human DLDH/DLDH/DLDH/DLD(NP_000099.2).

Conjugate: Unconjugated

Modification: Unmodified