DNA Ligase IV Polyclonal Antibody - BT-AP02654

DNA Ligase IV Polyclonal Antibody

Sizes: 20μL, 50μL, 100μL

Catalogue Numbers: BT-AP02654-20, BT-AP02654-50, BT-AP02654-100

Citations, Manuals and MSDS Available upon request.

Background: DNA ligase 4 encoded by LIG4 is a DNA ligase that joins single-strand breaks in a double-stranded polydeoxynucleotide in an ATP-dependent reaction. This protein is essential for V(D)J recombination and DNA double-strand break (DSB) repair through nonhomologous end joining (NHEJ). This protein forms a complex with the X-ray repair cross complementing protein 4 (XRCC4), and further interacts with the DNA-dependent protein kinase (DNA-PK). Both XRCC4 and DNA-PK are known to be required for NHEJ. The crystal structure of the complex formed by this protein and XRCC4 has been resolved. Defects in this gene are the cause of LIG4 syndrome. Alternatively spliced transcript variants encoding the same protein have been observed.

Research Areas: Epigenetics and Nuclear Signaling

Target Protein: LIG4

Applications: WB, IHC-p, IF, ELISA

Reactivity: Human

Clonality: Polyclonal

Host: Rabbit

Isotype: IgG

Full Product Name: DNA Ligase IV Antibody

Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Storage: -20°C for 1 year

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration: 1 mg/ml

Molecular Weight (Da): 103971

UniProt Accession: Human: P49917; Mouse: Q8BTF7

Synonyms: LIG4; DNA ligase 4; DNA ligase IV; Polydeoxyribonucleotide synthase [ATP] 4

GeneID: Human: 3981

Research Use Only