Product Description

DNASE1L1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS75205-50, BS75205-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P49184

Host: Rabbit

Reactivity: Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: This gene encodes a deoxyribonuclease protein that shows high sequence similarity to DNase I. The encoded protein is localized to the endoplasmic reticulum and modified by N-linked glycosylation. Alternate transcriptional splice variants encoding the same protein have been observed.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 34kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of mouse heart, using DNASE1L1 Antibody at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s., Immunofluorescence analysis of C6 cells using DNASE1L1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of NIH/3T3 cells using DNASE1L1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: DNASE1L1; DNAS1L1; DNASEX; DNL1L; G4.8; XIB

Immunogen: Recombinant fusion protein of human DNASE1L1(NP_006721.1).

Conjugate: Unconjugated

Modification: Unmodified