Dog β2-MG (Beta-2-Microglobulin) ELISA Kit
Sizes: 48T, 96T, 96T*5
Catalogue Numbers: ELK0336-48, ELK0336-96, ELK0336-5x96
Citations, Manuals and MSDS Available upon request.
Alternative Names: Beta 2M; BMG; B2MG; b2M; Beta-2-Microglobulin
Assay Type: Sandwich
Sensitivity: 0.23 µg/mL
Standard: 50 µg/mL
Detection Range: 0.78-50 µg/mL
Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
Assay Length: 3.5h
Research Areas: Infection immunity; Kidney biomarker;
Test Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog β2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog β2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog β2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog β2-MG in the samples is then determined by comparing the OD of the samples to the standard curve.
UniProt ID: P19341
Research Use Only