Product Description

E Cadherin Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66875-50, MB66875-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellμlar domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development . The classic cadherin subfamily includes N-, P-, R-, B-, and E-cadherins, as well as about ten other members that are found in adherens junctions, a cellμlar structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with 尾-catenin, 纬-catenin , and p120 catenin. 尾-catenin and 纬-catenin associate with 伪-catenin, which links the cadherin-catenin complex to the actin cytoskeleton . While 尾- and 纬-catenin play structural roles in the junctional complex, p120 regμlates cadherin adhesive activity and trafficking . Investigators consider E-cadherin an active suppressor of invasion and growth of many epithelial cancers . Research studies indicate that cancer cells have upregμlated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-cadherin cooperates with the FGF receptor, leading to overexpression of MMP-9 and cellμlar invasion . Research studies have shown that in endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascμlar permeability and tumor angiogenesis . Investigators have also demonstrated that expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers .

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB, IHC, IF/ICC

Alternate Names: CDHE, UVO, Cadherin-1, CAM 120/80, Epithelial cadherin, E-cadherin, Uvomorμlin, CD324

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human E Cadherin. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/2000), IHC (1/50 - 1/200), IF/ICC (1/10 - 1/50)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of E Cadherin protein.

Molecular Weight: ~ 130 kDa

Pathway: Adherens Junction signaling, ErbB HER Signaling, Wntβ catenin signaling

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P12830

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only