E2F-1 Polyclonal Antibody - BT-AP02789

E2F-1 Polyclonal Antibody

Sizes: 20μL, 50μL, 100μL

Catalogue Numbers: BT-AP02789-20, BT-AP02789-50, BT-AP02789-100

Citations, Manuals and MSDS Available upon request.

Background: The protein encoded by E2F1 (E2F transcription factor 1) is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Research Areas: Epigenetics and Nuclear Signaling

Target Protein: E2F1

Applications: IF, WB, IHC-p, ELISA

Reactivity: Human, Mouse, Rat

Clonality: Polyclonal

Host: Rabbit

Isotype: IgG

Full Product Name: E2F-1 Antibody

Immunogen: The antiserum was produced against synthesized peptide derived from human AMPK alpha around the phosphorylation site of Thr172. AA range:140-189

Storage: -20°C for 1 year

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Concentration: 1 mg/ml

Molecular Weight (Da): 46920

UniProt Accession: Human: Q01094; Mouse: Q61501; Rat: O09139

Synonyms: E2F1 RBBP3

GeneID: Human: 1869; Mouse: 13555

Research Use Only