Product Description

E2F1 Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS66106-50, BS66106-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regμlated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC-P, ICC/IF, IP, FC

Alternate Names: E2F 1, E2F transcription factor 1, E2F-1, E2f1 E2F transcription factor 1, KIAA4009, mKIAA4009, OTTHUMP00000030661, PBR 3, PBR3, PRB binding protein E2F 1, PRB-binding protein E2F-1, RBAP 1, RBAP-1, RBAP1, RBBP 3, RBBP-3, RBBP3, RBP 3, RBP3, Retinoblastoma associated protein 1, Retinoblastoma binding protein 3, Retinoblastoma-associated protein 1, Retinoblastoma-binding protein 3, Transcription factor E2F1, E2F1_HUMAN

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Unmodified

Immunogen: A synthesized peptide derived from human E2F1.

Dilution: WB: 1:500-1:1000
IHC-P: 1:50-1:100
ICC/IF: 1:50-1:100
IP: 1:20
FC: 1:50-1:100

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: E2F1 polyclonal antibody detects endogenous levels of E2F1 protein.

Molecular Weight: ~ 65 kDa

Pathway: ErbB HER Signaling，Cell Cycle Control G1 S Checkpoin

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: Q01094

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only