Product Description

EasyStep Rat T3 (Triiodothyronine) ELISA Kit

Sizes: 48T, 96T, 96T*5

Catalogue Numbers: ELK050ES-48, ELK050ES-96, ELK050ES-96*5

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Reactivity: Rat

Alternate Names: 3,3',5-Triiodo-L-Thyronine, Liothyronine, Cytomel, Tertroxin

Sensitivity: 0.31 ng/mL

Assay Type: Competitive Inhibition

Standard: 20 ng/mL

Detection Range: 0.32-20 ng/mL

Sample Type: Serum, Plasma

Assay Length: 1.5h

Research Area: Endocrinology, Autoimmunity

Test Principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat T3 antigen, and the Rat T3 standard plate wells that pre-coated using protein-related techniques are provided separately. Standard/Sample Diluent Buffer or samples are added to the appropriate microtiter plate wells ,then added a HRP-conjugated antibody specific to Rat T3 to each microplate well and incubated . After TMB substrate solution is added, the enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ±10nm. The concentration of Rat T3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Research Use Only