Product Description

ECA39 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES4173-50, ES4173-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: branched chain amino acid transaminase 1(BCAT1) Homo sapiens This gene encodes the cytosolic form of the enzyme branched-chain amino acid transaminase. This enzyme catalyzes the reversible transamination of branched-chain alpha-keto acids to branched-chain L-amino acids essential for cell growth. Two different clinical disorders have been attributed to a defect of branched-chain amino acid transamination: hypervalinemia and hyperleucine-isoleucinemia. As there is also a gene encoding a mitochondrial form of this enzyme, mutations in either gene may contribute to these disorders. Alternatively spliced transcript variants have been described. [provided by RefSeq, May 2010],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: BCAT1, BCT1, ECA39, Branched-chain-amino-acid aminotransferase, cytosolic, BCAT(c), Protein ECA39

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from the Internal region of human BCAT1. AA range:231-280

Isotype: IgG

Target: ECA39

Specificity: ECA39 Polyclonal Antibody detects endogenous levels of ECA39 protein.

Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: During embryogenesis, expressed in the brain and kidney, Overexpressed in MYC-induced tumors such as Burkitt's lymphoma,

Observed Band (KD): 43kD

Fields: Cysteine and methionine metabolism, Valine, leucine and isoleucine degradation, Valine, leucine and isoleucine biosynthesis, Pantothenate and CoA biosynthesis, Metabolic pathways, 2-Oxocarboxylic acid metabolism, Biosynthesis of amino acids, Biosynthesis of cofactors

Function: Catalytic Activity: 2-oxoglutaric acid + L-isoleucine = (S)-3-methyl-2-oxopentanoic acid + L-glutamic acid
Catalytic Activity: 2-oxoglutaric acid + L-valine = 3-methyl-2-oxobutanoic acid + L-glutamic acid
Catalytic Activity: L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate
Cofactor: Pyridoxal phosphate
Function: Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine
Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family
Subunit: Homodimer
Tissue Specificity: During embryogenesis, expressed in the brain and kidney. Overexpressed in C-myc induced tumors such as Burkitt's lymphoma

Subcellular Location: Cytoplasm

Storage and Stability: -20°C/1 year

Gene Name: BCAT1

Protein Name: Branched-chain-amino-acid aminotransferase, cytosolic

Mouse Gene ID: 12035

Mouse SwissProt Number: P24288

Rat Gene ID: 29592

Rat SwissProt Number: P54690

Research Use Only