Product Description

EF-1 α1/2 (Acetyl Lys41) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1103-50, ES1103-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes an isoform of the alpha subunit of the elongation factor-1 complex, which is responsible for the enzymatic delivery of aminoacyl tRNAs to the ribosome. This isoform (alpha 1) is expressed in brain, placenta, lung, liver, kidney, and pancreas, and the other isoform (alpha 2) is expressed in brain, heart and skeletal muscle. This isoform is identified as an autoantigen in 66% of patients with Felty syndrome. This gene has been found to have multiple copies on many chromosomes, some of which, if not all, represent different pseudogenes. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: EEF1A1, EEF1A, EF1A, LENG7, Elongation factor 1-alpha 1, EF-1-alpha-1, Elongation factor Tu, EF-Tu, Eukaryotic elongation factor 1 A-1, eEF1A-1, Leukocyte receptor cluster member 7, EEF1A2, EEF1AL, STN, Elongation factor 1-alpha 2, EF-1-alpha-2, Eukaryotic elongation factor 1 A-2, eEF1A-2, Statin-S1, EEF1A1P5, EEF1AL3, Putative elongation factor 1-alpha-like 3, EF-1-alpha-like 3, Eukaryotic elongation factor 1 A-like 3, eEF1A-like 3, Eukaryotic translation elongation factor 1 alpha-1 pseudogene 5

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized Acetyl-peptide derived from human EEF1A around the Acetylation site of Lys41. AA range:1-50

Isotype: IgG

Target: EF-1 α1/2

Specificity: Acetyl-EF-1 α1/2 (K41) Polyclonal Antibody detects endogenous levels of EF-1 α1/2 protein only when acetylated at K41.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle,

Observed Band (KD): 50kD

Fields: Nucleocytoplasmic transport, Legionellosis, Leishmaniasis

Function: Caution: Could be the product of a pseudogene
Function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis
Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily
Subunit: Found in a nuclear export complex with XPO5, EEF1A1, Ran and aminoacylated tRNA. Interacts with XPO5. May interact with ERGIC2
Tissue Specificity: Brain, placenta, lung, liver, kidney, pancreas but barely detectable in heart and skeletal muscle

Subcellular Location: Cytoplasm Nucleus Nucleus, nucleolus Cell membrane Colocalizes with DLC1 at actin-rich regions in the cell periphery (PubMed:19158340) Translocates together with ZPR1 from the cytoplasm to the nucleus and nucleolus after treatment with mitogens (PubMed:8650580) Localization at the cell membrane depends on EEF1A1 phosphorylation status and the presence of PPP1R16B (PubMed:26497934)

Storage and Stability: -20°C/1 year

Gene Name: EEF1A1/EEF1A2/EEF1A1P5

Protein Name: Elongation factor 1-alpha 1/Elongation factor 1-alpha 2/Putative elongation factor 1-alpha-like 3

Mouse Gene ID: 13627

Mouse SwissProt Number: P10126

Rat Gene ID: 171361

Rat SwissProt Number: P62630

Research Use Only