Product Description

EF-2 Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES2230-50, ES2230-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: This gene encodes a member of the GTP-binding translation elongation factor family. This protein is an essential factor for protein synthesis. It promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome. This protein is completely inactivated by EF-2 kinase phosporylation. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: EEF2, EF2, Elongation factor 2, EF-2

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human eEF2. AA range:31-80

Isotype: IgG

Target: eEF2

Specificity: EF-2 Polyclonal Antibody detects endogenous levels of EF-2 protein.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Brain, Cajal-Retzius cell, Epithelium, Hepatocyte, Ovary, Periph

Observed Band (KD): 100kD

Fields: AMPK signaling pathway, Oxytocin signaling pathway

Function: Function: This protein promotes the GTP-dependent translocation of the nascent protein chain from the A-site to the P-site of the ribosome
PTM: Diphthamide is 2-[3-carboxyamido-3-(trimethyl-ammonio)propyl]histidine. Diphthamide can be ADP-ribosylated by diphtheria toxin and by Pseudomonas exotoxin A
PTM: Phosphorylation by EF-2 kinase completely inactivates EF-2
Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily
Subunit: Component of the mRNA surveillance SURF complex, at least composed of ERF1, ERF3 (ERF3A or ERF3B), EEF2, UPF1/RENT1, SMG1, SMG8 and SMG9

Subcellular Location: Cytoplasm Nucleus Phosphorylation by CSK promotes cleavage and SUMOylation-dependent nuclear translocation of the C-terminal cleavage product

Storage and Stability: -20°C/1 year

Gene Name: EEF2

Protein Name: Elongation factor 2

Mouse Gene ID: 13629

Mouse SwissProt Number: P58252

Rat Gene ID: 29565

Rat SwissProt Number: P05197

Research Use Only