Product Description

EGFP Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65530-50, BS65530-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: P42212

Host: Rabbit

Reactivity: Recombinant EGFP

Applications: WB,

All Applications: WB=1:1000-10000
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.

Background: Enhanced green fluorescence protein (EGFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelenth of 509 nm) when excited by blue light (excitation peak at a wavelenth of 395 nm). EGFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. EGFP fluorescence is stable under fixation conditions and suitable for a variety of applications. EGFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize EGFP-tagged proteins within living cells without the need for chemical staining. Other applications of EGFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. EGFP technnology has considerably contributed to a greater understanding of cellular physiology.
Description: EGFP produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 239 amino acids and having a molecular mass of 27kDa. EGFP is purified by proprietary chromatographic techniques

Purification and Purity: affinity purified by Protein A

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: EGFP polyclonal Antibody detects endogenous levels of EGFP protein.

Bioworld Molecular Weight: ~28kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Anti-EGFP at 1/1000 ~ 1/5000 dilution, Anti-EGFP at 1/1000 ~ 1/5000 dilution, Anti-EGFP at 1/1000 dilution

Alternative Name: Enhanced Green Fluorecent protein; rEGFP; Recombinant EGFP; Enhanced Green Fluorecent protein.

Immunogen: Recombinant EGFP protin:

Conjugate: Unconjugated

Modification: Unmodified