Product Description

EGFR (Phospho-Y869) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS67432-50, BS67432-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding resμlts in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation . Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins . c-Src is involved in phosphorylation of EGFR at Tyr845 . The SH2 domain of PLCγ binds at phospho-Tyr992, resμlting in activation of PLCγ-mediated downstream signaling . Phosphorylation of EGFR at Tyr1045 creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation . The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068 . A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation . Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II, mutation of either of these serines resμlts in upregμlated EGFR tyrosine autophosphorylation.

Category: Primary Antibody

Reactivity: Human

Host: Rabbit

Applications: WB, IF/ICC

Alternate Names: ERBB, ERBB1, HER1, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human EGFR with a site at pY869. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000), IF/ICC (1/50 - 1/200)

Purification: The antibody was purified by immunogen affinity chromatography.

Specificity: Recognizes endogenous levels of EGFR with a site at pY869 protein.

Molecular Weight: ~ 175 kDa

Pathway: Tumor Angiogenesis，ErbB HER Signaling，Jak Stat: IL-6 Receptor Signaling.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P00533

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only