Product Description

eIF4A1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS7106-50, BS7106-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P60842

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IF/ICC, IP,

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:20 - 1:50 | IP,1:20 - 1:50

Background: ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 48KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of various lysates, using eIF4A1 antibody at 1:2170 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s., Immunofluorescence analysis of C6 cells using eIF4A1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of NIH/3T3 cells using eIF4A1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of U2OS cells using eIF4A1 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: EIF4A1; DDX2A; EIF-4A; EIF4A; eIF-4A-I; eIF4A-I

Immunogen: A synthetic peptide of human eIF4A1(NP_001407.1).

Conjugate: Unconjugated

Modification: Unmodified