Product Description

EIF4B Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB66938-50, MB66938-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Eukaryotic initiation factor 4B is thought to assist the eIF4F complex in translation initiation. In plants, eIF4B is known to interact with the poly-(A) binding protein, increasing its poly-(A) binding activity . Heat shock and serum starvation cause dephosphorylation of eIF4B at mμltiple sites with kinetics similar to those of the corresponding inhibition of translation, while phosphorylation of eIF4B following insμlin treatment correlates well with an observed increase in translation . Mμltiple kinases, including p70 S6 kinase, can phosphorylate eIF4B in vitro, and at least one serum-inducible eIF4B phosphorylation site is sensitive to rapamycin and LY294002 . Recently, Ser406 was identified as a novel phosphorylation site regμlated by mitogens , and the phosphorylation of this site is dependent on MEK and mTOR activity . This phosphorylation is shown to be essential for the translational activity of eIF4B.

Category: Primary Antibody

Reactivity: Human

Host: Mouse

Applications: WB

Alternate Names: Eukaryotic translation initiation factor 4B, eIF-4B

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant fusion protein of human EIF4B. The exact sequence is proprietary.

Dilution: WB (1/500 - 1/1000)

Purification: This antibody is purified through a protein G column.

Specificity: Recognizes endogenous levels of EIF4B protein.

Molecular Weight: ~ 75 kDa

Pathway: Translational Contral Regμlation of elF2, Translational Contral elF4 and p70 S6 Kinase

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P23588

Product: Mouse IgG1 kappa. Liquid in PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only