Product Description

eIF4E (phospho-S209) Polyclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: BS4748-50, BS4748-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: eIF4E, a protein modμlates translation of maternal mRNAs in early embryos before the onset of zygotic transcription. eIF4E also influences the overall rate of translation. eIF4E binds to the 7 methyl GTP cap structure of eukaryotic mRNAs. Phosphorylation of eIF4E on serine 209 regμlates the affinity of this protein for the 7 methyl GTP cap and/or RNA. Phosphorylation also enhances the interaction of eIF4E with eIF4G, which form a complex known as eIF4F. eIF4E phosphorylation is correlated with increased translational rate in a number of cell types. Several kinases are currently being investigated as potential regμlators of eIF4E including PKC and/or the MAP kinase activated Mnk.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC

Alternate Names: eIF4E ( phospho S209 ), eIF-4E, eIF4E, mRNA cap-binding protein, eIF-4F 25 kDa subunit, EIF4E, 1977, EIF4E, 133440, P06730, CBP, EIF4EL1, EIF4F, MGC111573,

Clonality: Polyclonal

Conjugate: Unconjugated

Modification: Phosphorylation

Immunogen: Synthetic phosphopeptide derived from human eIF4E around the phosphorylation site of Serine 209.

Dilution: WB: 1:500~1:1000
IHC: 1:50~1:200

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: p-eIF4E (S209) polyclonal antibody detects endogenous levels of eIF4E protein only when phosphorylated at Ser209.

Molecular Weight: ~ 25 kDa

Pathway: Insμlin &Glucose Signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P06730

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Research Use Only