EIF4E2 Polyclonal Antibody-BS70612

EIF4E2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS70612-50, BS70612-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O60573

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB,1:200 - 1:2000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200

Background: Recognizes and binds the 7-methylguanosine-containing mRNA cap during an early step in the initiation. Acts as a repressor of translation initiation. In contrast to EIF4E, it is unable to bind eIF4G (EIF4G1, EIF4G2 or EIF4G3, suggesting that it acts by competing with EIF4E and block assembly of eIF4F at the cap (By similarity. In P-bodies, component of a complex that promotes miRNA-mediated translational repression.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 27KDa/28KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using EIF4E2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 2s., Western blot analysis of extracts of various cell lines, using EIF4E2 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s., Immunofluorescence analysis of C6 cells using EIF4E2 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of NIH-3T3 cells using EIF4E2 Rabbit pAb at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: EIF4E2; 4E-LP; 4EHP; EIF4EL3; IF4e; h4EHP

Immunogen: Recombinant fusion protein of human EIF4E2(NP_004837.1).

Conjugate: Unconjugated

Modification: Unmodified