eIF5B (H1086) Polyclonal Antibody-BS3125

eIF5B (H1086) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS3125-50, BS3125-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: O60841

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: IHC

All Applications: IHC:1:50~1:200

Background: Accurate initiation of translation in eukaryotes is complex and requires many factors, some of which are composed of multiple subunits. The process is simpler in prokaryotes which have only three initiation factors (IF1, IF2, IF3). Two of these factors are conserved in eukaryotes: the homolog of IF1 is eIF1A and the homolog of IF2 is eIF5B. This gene encodes eIF5B. Factors eIF1A and eIF5B interact on the ribosome along with other initiation factors and GTP to position the initiation methionine tRNA on the start codon of the mRNA so that translation initiates accurately.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Immunohistochemistry (IHC) analyzes of eIF5B (H1086) pAb in paraffin-embedded human breast carcinoma tissue at 1:100.

Bioworld Molecular Weight: ~ 139 kDa

Specificity: eIF5B (H1086) polyclonal antibody detects endogenous levels of eIF5B protein.

Alternative Name: Eukaryotic translation initiation factor 5B; Translation initiation factor IF2; IF2; FLJ10524; KIAA0741; DKFZp434I036; Translation initiation factor IF 2; eIF 5B

Immunogen: Synthetic peptide, corresponding to amino acids 1061-1110 of Human eIF5B.

Conjugate: Unconjugated

Modification: Unmodified