EIF6 Polyclonal Antibody-BS6641

EIF6 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS6641-50, BS6641-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P56537

Host: Rabbit

Reactivity: Human

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: Hemidesmosomes are structures which link the basal lamina to the intermediate filament cytoskeleton. An important functional component of hemidesmosomes is the integrin beta-4 subunit (ITGB4), a protein containing two fibronectin type III domains. The protein encoded by this gene binds to the fibronectin type III domains of ITGB4 and may help link ITGB4 to the intermediate filament cytoskeleton. The encoded protein, which is insoluble and found both in the nucleus and in the cytoplasm, can function as a translation initiation factor and prevent the association of the 40S and 60S ribosomal subunits. Multiple non-protein coding transcript variants and variants encoding two different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 27kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using EIF6 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST., Immunofluorescence analysis of MCF-7 cells using EIF6 antibody. Blue: DAPI for nuclear staining., Confocal immunofluorescence analysis of Hela cells using EIF6 antibody at dilution of 1:50. Blue: DAPI for nuclear staining., Confocal immunofluorescence analysis of U-2OS cells using EIF6 antibody at dilution of 1:50. Blue: DAPI for nuclear staining.

Alternative Name: EIF6; CAB; EIF3A; ITGB4BP; b(2)gcn; eIF-6; p27(BBP); p27BBP

Immunogen: Recombinant fusion protein of human EIF6(NP_852133.1).

Conjugate: Unconjugated

Modification: Unmodified