ELISA Kit for Complement Factor H (CFH)
Size: 96T
Catalogue Number: UCEA635Ra-96
Citations, Manuals and MSDS Available upon request.
Target: Complement Factor H (CFH)
Alternative Names: CF-H; FH; FHL1; ARMD4; ARMS1; CFHL3; HF1; HF2; HUS; H Factor 2; Age-Related Maculopathy Susceptibility 1; Adrenomedullin binding protein
Target Species: Rat
Detection range: 12.35-1,000ng/mL
Sensitivity: 5.77ng/mL
Uniprot: G3V9R2
Gene ID: 155012
Featured Series Function: Detects small molecule
Recommended/Predicted Sample Types: Serum, Plasma and other Biological Fluids
Assay Time: 2h
Method: Colormetric
Specificity: Reactive with Rat CFH / Complement Factor H
Detection principle: Competitive Inhibition
Sample Size: 50uL
Assay Precision: Intra-Assay: CV<10%, Inter-Assay: CV<12%
Storage: 4°C for 1 month/ -20°C for long-term(One year within shelf life)
Shelf-life: 12 months
Specificity: This assay has high sensitivity and excellent specificity for detection of Complement Factor H (CFH).
No significant cross-reactivity or interference between Complement Factor H (CFH) and analogues was observed.
Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Complement Factor H (CFH) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Complement Factor H (CFH) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Stability: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Assay procedure summary: 1. Prepare all reagents, samples and standards;
2. Add 50µL standard or sample to each well.
And then add 50µL prepared Detection Reagent A immediately.
Shake and mix. Incubate 1 hour at 37°C;
3. Aspirate and wash 5 times;
4. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C;
5. Add 50µL Stop Solution. Read at 450 nm immediately.
Test principle: The microtiter plate provided in this kit has been pre-coated with an antigen. Standards or samples are then added to the appropriate microtiter plate wells with a Horseradish Peroxidase (HRP)-conjugated secondary antibody. After TMB substrate solution is added, those wells that contain Complement Factor H (CFH) will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Complement Factor H (CFH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Research Area: Metabolic pathway; Infection immunity; Immune molecule;
Research Use Only