Product Description

ELSPBP1 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS76519-50, BS76519-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q96BH3

Host: Rabbit

Reactivity: Mouse, Rat

Applications: WB, IF/ICC

All Applications: WB,1:500 - 1:2000 | IF/ICC,1:50 - 1:200

Background: The protein encoded by this gene belongs to the sperm-coating protein family of epididymal origin. This protein and its canine homolog are the first known examples of proteins with four tandemly arranged fibronectin type 2 (Fn2) domains in the Fn2-module protein family.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 26kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using ELSPBP1 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s., Immunofluorescence analysis of rat testis using ELSPBP1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining., Immunofluorescence analysis of mouse testis using ELSPBP1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: ELSPBP1; E12; EDDM12; EL149; HE12

Immunogen: Recombinant fusion protein of human ELSPBP1(NP_071425.3).

Conjugate: Unconjugated

Modification: Unmodified