Product Description

Emerin/EMD Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS75527-50, BS75527-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P50402

Host: Rabbit

Reactivity: Human, Mouse

Applications: WB, IHC, IF/ICC

All Applications: WB,1:500 - 1:2000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200

Background: Emerin is a serine-rich nuclear membrane protein and a member of the nuclear lamina-associated protein family. It mediates membrane anchorage to the cytoskeleton. Dreifuss-Emery muscular dystrophy is an X-linked inherited degenerative myopathy resulting from mutation in the emerin gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Unmodification

Bioworld Molecular Weight: 35kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using Emerin/Emerin/EMD antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST., Immunohistochemistry of paraffin-embedded human gastric cancer using Emerin/Emerin/EMD antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded human uterine cancer using Emerin/Emerin/EMD antibody at dilution of 1:100. Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol., Immunofluorescence analysis of U-2 OS cells using Emerin/Emerin/EMD antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: EMD; EDMD; LEMD5; STA; emerin

Immunogen: Recombinant fusion protein of human Emerin/Emerin/EMD(NP_000108.1).

Conjugate: Unconjugated

Modification: Unmodified