Product Description

Endonuclease G Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: CAS75127-50, CAS75127-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.4.

Swiss-Prot:  Q14249

Host: Rabbit

Reactivity: Human, Rat, Mouse

Applications: WB, IP, IHC

All Applications: WB: 1:2000~1:5000
IP:1:2000~8000
IHC:1:50~1:100

Background: Endonuclease G (EndoG) is a nuclear-encoded mitochondrial nuclease that has been reported to function in apoptosis, DNA recombination and cell proliferation. EndoG is expressed as a precursor protein containing an amino-terminal mitochondrial targeting sequence which is removed when the protein is imported into the mitochondria. During apoptosis EndoG is released from the mitochondria, translocates to the nucleus and cooperates with other nucleases to trigger DNA fragmentation associated with the apoptotic process

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Endonuclease G polyclonal antibody detects endogenous levels of Endonuclease G protein.

Bioworld Molecular Weight: ~ 30 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of Endonuclease G polyclonal antibody at 1:5000 dilution
Lane 1: K562 whole cell lysate (40ug)
Lane 2: U-87MG whole cell lysate (40ug)
Lane 3: HepG2 whole cell lysate (40ug)
Lane 4: The Heart tissue lysate of Mouse (40ug)
Lane 5: The Muscle tissue lysate of RAT (40ug), Immunoprecipitation - Endonuclease G Polyclonal Antibody, Immunohistochemistry of paraffin-embedded human colon carcinoma using Endonuclease G antibody at dilution of 1:50., Immunohistochemistry of paraffin-embedded human colon carcinoma using Endonuclease G antibody at dilution of 1:50.

Alternative Name: Endonuclease G, mitochondrial ; Endo G; ENDOG

Immunogen: Recombinant protein of human Endonuclease G.

Conjugate: Unconjugated

Modification: Unmodified