Product Description

ENOA Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES11763-50, ES11763-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: enolase 1(ENO1) Homo sapiens This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy. [provided by RefSeq, Jan 2011],

Reactivity: Human, Rat, Mouse,

Host: Rabbit

Applications: WB, ELISA

Clonality: Polyclonal

Immunogen: Synthesized peptide derived from part region of human protein AA range: 120-170

Isotype: IgG

Target: ENOA

Specificity: ENOA Polyclonal Antibody detects endogenous levels of protein.

Dilution: WB 1:500-2000 ELISA 1:5000-20000

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, and 0.02% sodium azide.

Expression: The alpha/alpha homodimer is expressed in embryo and in most adult tissues, The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons,

Observed Band (KD): 47kD

Fields: Glycolysis / Gluconeogenesis, Metabolic pathways, Carbon metabolism, Biosynthesis of amino acids, RNA degradation, HIF-1 signaling pathway

Function: Catalytic Activity: 2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O
Cofactor: Magnesium. Required for catalysis and for stabilizing the dimer
Developmental Stage: During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells
Disease: Antibodies against alpha-enolase are present in sera from patients with cancer-associated retinopathy syndrome (CAR), a progressive blinding disease which occurs in the presence of systemic tumor growth, primarily small-cell carcinoma of the lung and other malignancies
Disease: ENO1 is identified as an autoantigen in Hashimoto encephalopathy (HE) a rare autoimmune disease associated with Hashimoto thyroiditis (HT). HT is a disorder in which destructive processes overcome the potential capacity of thyroid replacement leading to hypothyroidis

Subcellular Location: Cytoplasm Cell membrane Cytoplasm, myofibril, sarcomere, M line Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form ENO1 is localized to the M line, [Isoform MBP-1]: Nucleus

Storage and Stability: -20°C/1 year

Gene Name: ENO1 ENO1L1 MBPB1 MPB1

Protein Name: Alpha-enolase (EC 4.2.1.11) (2-phospho-D-glycerate hydro-lyase) (C-myc promoter-binding protein) (Enolase 1) (MBP-1) (MPB-1) (Non-neural enolase) (NNE) (Phosphopyruvate hydratase) (Plasminogen-binding

Mouse SwissProt Number: P17182

Rat SwissProt Number: P04764

Research Use Only