Product Description

eNOS Monoclonal Antibody

Sizes: 50μl, 100μl

Catalogue Numbers: MB65920-50, MB65920-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascμlar system. It catalyzes the production of nitric oxide (NO), a key regμlator of blood pressure, vascμlar remodeling, and angiogenesis. The activity of eNOS is regμlated by phosphorylation at mμltiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495. Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimμli. In contrast, bradykinin and H2O2 activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation.

Category: Primary Antibody

Reactivity: Human, Mouse, Rat

Host: Mouse

Applications: WB, IHC

Alternate Names: Nitric oxide synthase endothelial, Constitutive NOS, cNOS, EC-NOS, Endothelial NOS, eNOS, NOS type III, NOSIII

Clonality: Monoclonal

Conjugate: Unconjugated

Modification: Unmodification

Immunogen: Recombinant protein corresponding to human eNOS.

Dilution: WB (1/500 - 1/2000), IHC (1/200 - 1/500)

Purification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: Recognizes endogenous levels of eNOS protein.

Molecular Weight: ~ 133 kDa

Pathway: AMPK Signaling Pathway, PI3K AKT signaling,

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

SwissProt: P29474

Product: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.

Research Use Only