Product Description

ENTPD1/CD39 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS70562-50, BS70562-100

Product: 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: P49961

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB, IHC, IF/ICC

All Applications: WB,1:200 - 1:1000 | IHC,1:50 - 1:200 | IF/ICC,1:50 - 1:200

Background: The protein encoded by this gene is a plasma membrane protein that hydrolyzes extracellular ATP and ADP to AMP. Inhibition of this protein's activity may confer anticancer benefits. Several transcript variants encoding different isoforms have been found for this gene.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Polyclonal Antibodies

Bioworld Molecular Weight: 80KDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of various cell lines, using ENTPD1/CD39 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG at 1:10000 dilution.
Lysates/proteins: 25ug per Lane.
Blocking buffer: 3% non-fat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 180s., Immunohistochemistry of paraffin-embedded human liver using ENTPD1/CD39 Rabbit pAb at dilution of 1:25. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunohistochemistry of paraffin-embedded human placenta using ENTPD1/CD39 Rabbit pAb at dilution of 1:25. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol., Immunofluorescence analysis of Raw264.7 cells using ENTPD1/CD39 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.

Alternative Name: ENTPD1; ATPDase; CD39; NTPDase-1; SPG64

Immunogen: Recombinant fusion protein of human ENTPD1/CD39(NP_001157655.1).

Conjugate: Unconjugated

Modification: Unmodified