Product Description

Ephrin-B1/2 (phospho Tyr330) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1419-50, ES1419-100

Lead times: 1-2 weeks, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene is a type I membrane protein and a ligand of Eph-related receptor tyrosine kinases. It may play a role in cell adhesion and function in the development or maintenance of the nervous system. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, ELISA

Alternate Names: EFNB1, EFL3, EPLG2, LERK2, Ephrin-B1, EFL-3, ELK ligand, ELK-L, EPH-related receptor tyrosine kinase ligand 2, LERK-2, EFNB2, EPLG5, HTKL, LERK5, Ephrin-B2, EPH-related receptor tyrosine kinase ligand 5, LERK-5, HTK ligand, HTK-L

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human EFNB1/2 around the phosphorylation site of Tyr330. AA range:284-333

Isotype: IgG

Target: Ephrin-B1/2

Specificity: Phospho-Ephrin-B1/2 (Y330) Polyclonal Antibody detects endogenous levels of Ephrin-B1/2 protein only when phosphorylated at Y330.

Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formlation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Widely expressed (PubMed:8070404, PubMed:7973638), Detected in both neuronal and non-neuronal tissues (PubMed:8070404, PubMed:7973638), Seems to have particularly strong expression in retina, sciatic nerve, heart and spinal cord (PubMed:7973638),

Observed Band (KD): 59kD

Fields: Axon guidance

Function: Disease: Defects in EFNB1 are a cause of craniofrontonasal syndrome (CFNS) [MIM: 304110], also known as craniofrontonasal dysplasia (CFND). CFNS is an X-linked inherited syndrome characterized by hypertelorism, coronal synostosis with brachycephaly, downslanting palpebral fissures, clefting of the nasal tip, joint anomalies, longitudinally grooved fingernails and other digital anomalies
Function: Binds to the receptor tyrosine kinases EPHB1 and EPHA1. Binds to, and induce the collapse of, commissural axons/growth cones in vitro. May play a role in constraining the orientation of longitudinally projecting axons
Induction: By TNF-alpha
PTM: Inducible phosphorylation of tyrosine residues in the cytoplasmic domain
Similarity: Belongs to the ephrin family
Subunit: Interacts with GRIP1 and GRIP2
Tissue Specificity: Heart, placenta, lung, liver, skeletal muscle, kidney, pancreas

Subcellular Location: Cell membrane, Single-pass type I membrane protein Membrane raft May recruit GRIP1 and GRIP2 to membrane raft domains, [Ephrin-B1 C-terminal fragment]: Cell membrane, Single-pass type I membrane protein, [Ephrin-B1 intracellular domain]: Nucleus Colocalizes with ZHX2 in the nucleus

Storage and Stability: -20°C/1 year

Gene Name: EFNB1/EFNB2

Protein Name: Ephrin-B1/Ephrin-B2

Mouse Gene ID: 13641/13642

Rat SwissProt Number: P52796

Research Use Only