Product Description

Epsin 3 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65369-50, BS65369-100

Product: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Swiss-Prot: Q9H201

Host: Rabbit

Reactivity: Human

Applications: WB, IHC-P, IF (paraffin section), ELISA

All Applications: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.

Background: induction:In keratinocytes, by wounding or contact with collagen.,similarity:Belongs to the epsin family.,similarity:Contains 1 ENTH (epsin N-terminal homology) domain.,similarity:Contains 2 UIM (ubiquitin-interacting motif) repeats.,subcellular location:Concentrated in the perinuclear region and associated with clathrin-coated vesicles close to the cell periphery. May shuttle to the nucleus.,tissue specificity:Detected in migrating keratinocytes from wounded skin, but not in differentiating keratinocytes or in normal skin. Detected in chronic wounds, basal cell carcinoma and ulcerative colitis.,

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: Epsin 3 Polyclonal Antibody detects endogenous levels of Epsin 3 protein.

Bioworld Molecular Weight: ~68 kDa

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of lysates from K562 cells, using EPN3 Antibody. The Lane on the right is blocked with the synthesized peptide., Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue, using EPN3 Antibody. The picture on the right is blocked with the synthesized peptide., Western blot analysis of the lysates from COLO205 cells using EPN3 antibody.

Alternative Name: EPN3; Epsin-3; EPS-15-interacting protein 3

Immunogen: The antiserum was produced against synthesized peptide derived from human EPN3. AA range:401-450

Conjugate: Unconjugated

Modification: Unmodified