Product Description

ERK 1/2 (phospho Tyr204) Rabbit Polyclonal Antibody

Sizes: 50μL, 100μL

Catalogue Numbers: ES1309-50, ES1309-100

Lead times: approximately 7-10 business days, if manufacturer has product in stock

Manufacturer/Ship Location: China

Background: The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq, Jul 2008],

Reactivity: Human, Mouse, Rat

Host: Rabbit

Applications: WB, IHC, IF, ELISA

Alternate Names: MAPK3; ERK1; PRKM3; Mitogen-activated protein kinase 3; MAP kinase 3; MAPK 3; ERT2; Extracellular signal-regulated kinase 1; ERK-1; Insulin-stimulated MAP2 kinase; MAP kinase isoform p44; p44-MAPK; Microtubule-associated protein 2 kinase; p

Clonality: Polyclonal

Immunogen: The antiserum was produced against synthesized peptide derived from human p44/42 MAP Kinase around the phosphorylation site of Tyr204. AA range:170-219

Isotype: IgG

Target: ERK 1/2,

Specificity: Phospho-ERK 1/2 (Y204) Polyclonal Antibody detects endogenous levels of ERK 1/2 protein only when phosphorylated at Y204.

Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration: 1 mg/ml,

Formulation: Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Expression: Epithelium, Eye, Hepatoma, Human cervix, Lymph,

Observed Band (KD): 44+42kD

Fields: EGFR tyrosine kinase inhibitor resistance, Endocrine resistance, Platinum drug resistance, MAPK signaling pathway, ErbB signaling pathway, Ras signaling pathway, Rap1 signaling pathway, cGMP-PKG signaling pathway, cAMP signaling pathway, Chemokine signaling pathway, HIF-1 signaling pathway, FoxO signaling pathway, Sphingolipid signaling pathway, Phospholipase D signaling pathway, Oocyte meiosis, Autophagy - animal, mTOR signaling pathway, PI3K-Akt signaling pathway, Apoptosis, Cellular senescence, Adrenergic signaling in cardiomyocytes, Vascular smooth muscle contraction, TGF-beta signaling pathway, Axon guidance, VEGF signaling pathway, Apelin signaling pathway, Osteoclast differentiation, Focal adhesion, Adherens junction, Gap junction, Signaling pathways regulating pluripotency of stem cells, Platelet activation, Neutrophil extracellular trap formation, Toll-like receptor signaling pathway, NOD-like receptor signaling pathway, C-type lectin recep

Function: Catalytic Activity: ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium. Domain: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases. enzyme regulation:Activated by tyrosine phosphorylation in response to insulin and NGF. Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4). PTM: Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme. Similarity: Belongs to the protein kinase superfamily. Similarity: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinas

Subcellular Location: Cytoplasm Nucleus. Membrane, caveola Cell junction, focal adhesion Autophosphorylation at Thr-207 promotes nuclear localization (PubMed:19060905). PEA15-binding redirects the biological outcome of MAPK3 kinase-signaling by sequestering MAPK3 into the cytoplasm (By similarity).

Storage and Stability: -20°C/1 year

Gene Name: MAPK1/MAPK3

Protein Name: Mitogen-activated protein kinase 3

Human Gene ID: 5595/5594

Human SwissProt Number: P27361/P28482

Mouse Gene ID: 26417/26413

Rat Gene ID: 50689/116590

Rat SwissProt Number: P21708/P63086

Research Use Only