Product Description

ERK1 + ERK2 Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS65610-50, BS65610-100

Product: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

Swiss-Prot: P63085

Host: Rabbit

Reactivity: Human, Mouse, Rat, (predicted: Chicken, Dog, Pig, Cow, Horse, Rabbit, )

Applications: WB, ，ICC，

All Applications: WB=1:500-2000 ICC=1:100

Background: The protein encoded by this gene is a member of the MAPkinase family. MAP kinases, also known as extracellularsignal-regulated kinases (ERKs), act in a signaling cascade thatregulates various cellular processes such as proliferation,differentiation, and cell cycle progression in response to avariety of extracellular signals. This kinase is activated byupstream kinases, resulting in its translocation to the nucleuswhere it phosphorylates nuclear targets. Alternatively splicedtranscript variants encoding different protein isoforms have beendescribed. [provided by RefSeq, Jul 2008].

Purification and Purity: affinity purified by Protein A

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Specificity: ERK1 + ERK2 Polyclonal Antibody detects endogenous levels of ERK1 + ERK2 protein.

Bioworld Molecular Weight: 42/44kD

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Sample:
A431(Human) Cell Lysate at 30 ug
Primary: Anti-ERK1 + ERK2 at 1/1000 dilution, Sample: A549 Cell Lysate at 30 ug
Primary: Anti- ERK1+ERK2 at 1/300 dilution, Tissue/cell: HUVEC cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum, C-0005) at 37°C for 20 min; Antibody incubation with (ERK1 + ERK2) Polyclonal Antibody, Unconjugated 1:100, 90 minutes at 37°C; followed by a conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue,) was used to stain the cell nuclei.

Alternative Name: ERK1/2; ERK1+ERK2; ERK1/ERK2; ERK 1; ERK 2; ERK-2; ERK1; ERK2; ERT1; ERT2; Extracellular signal regulated kinase 1; Extracellular signal regulated kinase 2; Extracellular signal-regulated kinase 2; HS44KDAP; HUMKER1A; Insulin stimulated MAP2 kinase; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; MAP kinase isoform p44; MAPK 1; MAPK 2; MAPK 3; MAPK1; MAPK2; MAPK3; MGC20180; Microtubule associated protein 2 kinase; Mitogen activated protein kinase 1; Mitogen activated protein kinase 2; Mitogen activated protein kinase 3; Mitogen-activated protein kinase 1; Mitogen-activated protein kinase 2; MK03_HUMAN; MK01_HUMAN; p38; p40; p41; p41mapk; p42 MAPK; p42-MAPK; p42MAPK; p44 ERK1; p44 MAPK; p44ERK1; p44MAPK; PRKM 1; PRKM 2; PRKM 3; PRKM1; PRKM2; PRKM3; Protein kinase mitogen activated 1; Protein kinase mitogen activated 2; Protein kinase mitogen activated 3; Protein tyrosine kinase ERK 2.

Immunogen: KLH conjugated synthetic peptide derived from human ERK1/2:251-358/358

Conjugate: Unconjugated

Modification: Unmodified