ERK3 (phospho-S189) Polyclonal Antibody-BS6377

ERK3 (phospho-S189) Polyclonal Antibody

Sizes: 50µl, 100µl

Catalogue Numbers: BS6377-50, BS6377-100

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Swiss-Prot: Q16659

Host: Rabbit

Reactivity: Human, Mouse, Rat

Applications: WB

All Applications: WB: 1:500~1:2000

Background: Mitogen-activated protein kinase (MAPK) signaling pathways involve closely related MAP kinases, including extracellular-signal-related kinase 3 (ERK 3, also designated PRKM6 and p97MAPK). Serum, growth factors and phorbol esters can initiate ERK 3 signaling pathways. Despite lacking a definitive nuclear localization sequence, ERK 3 constitutively localizes to the nucleus upon activation. p38 pathway activation-dependent upregulation of ERK 3 is independent of the status of p53, Bcl2 and caspase 3 during cell stress and damage induced by proteasome inhibition, suggesting ERK 3 in part mediates intracellular defense or cell rescue. The human ERK3 gene maps to chromosome 15q21 and encodes a 721 amino acid protein.

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot analysis of extracts of 293 cells, using Phospho-ERK3-S189 antibody.

Bioworld Molecular Weight: ~ 120 kDa

Specificity: P-ERK3(S189) polyclonal antibody detects endogenous levels of ERK3 protein only when phosphorylated at Ser189.

Alternative Name: Mitogen-activated protein kinase 6; MAP kinase 6; MAPK 6; Extracellular signal-regulated kinase 3; ERK-3; MAP kinase isoform p97; p97-MAPK; MAPK6; ERK3; PRKM6

Immunogen: Synthetic phosphopeptide derived from human ERK3 around the phosphorylation site of Serine 189.

Conjugate: Unconjugated

Modification: Phosphorylation