ESPL1 Rabbit pAb
Sizes: 20μL, 100μL
Catalogue Numbers: A15366-20, A15366-100
Citations, Manuals and MSDS Available upon request.
Background: Stable cohesion between sister chromatids before anaphase and their timely separation during anaphase are critical for chromosome inheritance. In vertebrates, sister chromatid cohesion is released in 2 steps via distinct mechanisms. The first step involves phosphorylation of STAG1 (MIM 604358) or STAG2 (MIM 300826) in the cohesin complex. The second step involves cleavage of the cohesin subunit SCC1 (RAD21; MIM 606462) by ESPL1, or separase, which initiates the final separation of sister chromatids (Sun et al., 2009 [PubMed 19345191]).
Category: Polyclonal Antibodies
Applications: WB, ELISA
Cross-reactivity: Human, Mouse, Rat
Protein Weight: 233kDa
Observed Molecular Weight: 233kDa
Immunogen: Recombinant protein (or fragment)
Species: Human
GeneID: 9700
SWISS: Q14674
Alternate Names: ESP1; SEPA; ESPL1
Source: Rabbit
Isotype: IgG
Purity: Affinity purification
Storage: Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH 7.3.
Recommended Dilutions: WB, 1:200 - 1:2000 ELISA, Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Research Areas: Epigenetics Nuclear Signaling, Protein phosphorylation, Cell Biology Developmental Biology, Apoptosis, Cell Cycle.
NCBI Alias: ESPL1
Research Use Only